November 15-22, 2009

Ljubljana, Slovenia

Electroporation based Technologies and Treatments



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and try out DEMO of EBTT distant learning!

Key lectures and content of the course:

Cells and tissues in electric fields

D. Miklavčič

  • Resting and induced transmembrane voltage
  • Dielectric properties of biological tissues
  • Temperature and conductivity changes in tissues
  • Physical Chemistry of Membrane Electroporation

    E. Neumann

  • Electrochemical membrane thermodynamics
  • Transport mechanisms
  • Electroporation in vitro and in vivo - protocols

    L.M. Mir

  • Electroporation of cells in vitro
  • Electroporation of cells in vivo
  • Parameters that determine the efficiency of electroporation
  • Development of devices and electrodes

    D. Miklavčič

  • Devices for in vitro and in vivo experimental work
  • Electrodes for in vitro and in vivo
  • Electroporation of cells in tissue - models of electroporation in vivo and their experimental validations
  • Electrochemotherapy of tumors

    G. Serša

  • Basic mechanisms of electrochemotherapy
  • Preclinical studies
  • Clinical use of electrochemotherapy
  • Future perspectives and current developments
  • Application of electroporation in gene transfection

    J. Teissié

  • Gene transfection in vitro
  • Gene transfection in vivo
  • Gene expression regulation by siRNA electrotransfer
  • Clinical use in veterinary medicine
  • Clinical use in human medicine
  • Electroporation in transdermal drug delivery

    V. Préat

  • Skin electroporation
  • Physical methodes for DNA delivery into skin
  • Additional topics:

  • Delivery of ultra-short, high-intensity pulsed electrical energy to membranes, cells, and tissues - biophysics and therapeutics
  • Safety issues and toxicity of in vivo electroporation
  • Principles and applications of non-thermal irreversible electroporation
  • Electroporation in food sanitization
  • Nanoelectropores of the cell plasma membrane
  • Molecular dynamics simulations of lipid bilayers electroporation
  • Laboratory and practical work

    M. Kandušer

  • Monitoring cell membrane electropermeabilization with ratiometric fluorescence dye FURA-2AM
  • Electroporation detection with propidium iodide
  • Gene electrotransfer using different electric pulses and/or electric field orientations
  • Electroporation of planar lipid bilayer
  • Molecular dynamics simulations
  • Electrochemotherapy treatment planning: optimization of voltage and electrode position
  • Numerical modeling of electric field in a cell suspension
  • Electrochemotherapy - interactive learning