November 15-22, 2009

Ljubljana, Slovenia

Electroporation based Technologies and Treatments

International SCIENTIFIC WORKSHOP and POSTGRADUATE COURSE

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Key lectures and content of the course:

Cells and tissues in electric fields

D. Miklavčič
  • Resting and induced transmembrane voltage
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  • Dielectric properties of biological tissues
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  • Temperature and conductivity changes in tissues
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    Physical Chemistry of Membrane Electroporation

    E. Neumann
  • Electrochemical membrane thermodynamics
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  • Transport mechanisms
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    Electroporation in vitro and in vivo - protocols

    L.M. Mir
  • Electroporation of cells in vitro
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  • Electroporation of cells in vivo
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  • Parameters that determine the efficiency of electroporation
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    Development of devices and electrodes

    D. Miklavčič
  • Devices for in vitro and in vivo experimental work
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  • Electrodes for in vitro and in vivo
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  • Electroporation of cells in tissue - models of electroporation in vivo and their experimental validations
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    Electrochemotherapy of tumors

    G. Serša
  • Basic mechanisms of electrochemotherapy
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  • Preclinical studies
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  • Clinical use of electrochemotherapy
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  • Future perspectives and current developments
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    Application of electroporation in gene transfection

    J. Teissié
  • Gene transfection in vitro
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  • Gene transfection in vivo
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  • Gene expression regulation by siRNA electrotransfer
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  • Clinical use in veterinary medicine
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  • Clinical use in human medicine
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    Electroporation in transdermal drug delivery

    V. Préat
  • Skin electroporation
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  • Physical methodes for DNA delivery into skin
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    Additional topics:
  • Delivery of ultra-short, high-intensity pulsed electrical energy to membranes, cells, and tissues - biophysics and therapeutics
    		•

  • Safety issues and toxicity of in vivo electroporation
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  • Principles and applications of non-thermal irreversible electroporation
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  • Electroporation in food sanitization
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  • Nanoelectropores of the cell plasma membrane
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  • Molecular dynamics simulations of lipid bilayers electroporation
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    Laboratory and practical work

    M. Kandušer
  • Monitoring cell membrane electropermeabilization with ratiometric fluorescence dye FURA-2AM
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  • Electroporation detection with propidium iodide
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  • Gene electrotransfer using different electric pulses and/or electric field orientations
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  • Electroporation of planar lipid bilayer
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  • Molecular dynamics simulations
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  • Electrochemotherapy treatment planning: optimization of voltage and electrode position
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  • Numerical modeling of electric field in a cell suspension
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  • Electrochemotherapy - interactive learning
    		•