EBTT 2009
Archived edition

EBTT 2009

Electroporation-Based Technologies and Treatments — International Scientific Workshop & Postgraduate Course

15–22 November 2009Ljubljana, Slovenia5 ECTS

The meeting

Cell membrane electroporation — mechanisms & applications

Cell membrane electroporation, its mechanisms and applications in biology, medicine and biotechnology. The basis of the phenomena, current applications and future prospects were the main topics of this international meeting.

Whether you were a postgraduate student or just wanted to learn and contribute to the discussion, you were kindly invited to attend the workshop and the course on electroporation technologies and treatments. Abstract submission was strongly encouraged and was necessary if credits (5 ECTS) were sought. Each participant submitting an abstract also presented it in the form of a short oral presentation.

Organised by the University of Ljubljana and the Institute of Oncology, Ljubljana. Organising committee chair: Peter Kramar. Members: Alenka Maček Lebar, Maša Kandušer, Matej Kranjc, Tadej Kotnik, Gorazd Pucihar, Mojca Pavlin, Nataša Pavšelj, Anže Županič.

Course content

Key lectures & topics

Cells and tissues in electric fields

D. Miklavčič — resting & induced transmembrane voltage; dielectric properties; temperature & conductivity changes.

Physical chemistry of membrane electroporation

E. Neumann — electrochemical membrane thermodynamics; transport mechanisms.

Electroporation in vitro & in vivo — protocols

L. M. Mir — electroporation of cells in vitro and in vivo; efficiency parameters.

Development of devices & electrodes

D. Miklavčič — devices & electrodes for in vitro/in vivo; tissue models and their validation.

Electrochemotherapy of tumours

G. Serša — mechanisms, preclinical & clinical use, future perspectives.

Application of electroporation in gene transfection

J. Teissié — gene transfection in vitro/in vivo; siRNA electrotransfer; veterinary & human clinical use.

Electroporation in transdermal drug delivery

V. Préat — skin electroporation; physical methods for DNA delivery into skin.

Additional topics

Nanosecond pulses; safety & toxicity of in-vivo EP; non-thermal irreversible electroporation; food sanitization; nanoelectropores; MD simulations of lipid bilayer electroporation.

Faculty

Directors & lecturers

Co-DirectorDamijan Miklavčič
Co-DirectorLluis M. Mir

Faculty members & lectures

  • Damijan MiklavčičUniversity of Ljubljana, Slovenia
  • Lluis M. MirCNRS & Institut Gustave Roussy, Villejuif, France
  • Eberhard NeumannUniversity of Bielefeld, Germany
  • Véronique PréatCatholic University of Louvain, Brussels, Belgium
  • Gregor SeršaInstitute of Oncology, Ljubljana, Slovenia
  • Justin TeissiéCNRS & IPBS, Toulouse, France

Invited lecturers

  • Maja ČemažarInstitute of Oncology, Ljubljana, Slovenia
  • Giovana FerrariUniversity of Salerno, Italy
  • Julie GehlCopenhagen University Hospital Herlev, Denmark
  • Muriel GolzioCNRS & IPBS, Toulouse, France
  • Tadej KotnikUniversity of Ljubljana, Slovenia
  • Malgorzata KotulskaWroclaw University of Technology, Poland
  • Andrei PakhomovOld Dominion University, Norfolk, USA
  • Marie-Pierre RolsCNRS & IPBS, Toulouse, France
  • Boris RubinskyUniversity of California at Berkeley, USA
  • Mounir TarekUniversité Henri-Poincaré, Nancy, France
  • P. Thomas VernierUniversity of Southern California, USA

Programme & schedule

Schedule downloads

Hands-on

Practical work & exercises

Wet-lab, computer-modelling and e-learning sessions. Participants could select up to three practical-work sessions. Exercise sheets (PDF) as distributed to participants. See also the practical-work assignment form.

S1Laboratory safetyPDF ↓
L1Gene electrotransfer with different pulse parametersPDF ↓
L2Monitoring cell membrane electroporation with ratiometric fluorescent dye Fura-2AMPDF ↓
L3Electropermeabilization detection of attached cells with propidium iodidePDF ↓
L4Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPSPDF ↓
L5Electroporation of planar lipid bilayersPDF ↓
L6Cell electrofusionPDF ↓
C1Electrochemotherapy treatment planning: optimization of voltage & electrode positionPDF ↓
C2Numerical modelling of coupled physical phenomenaPDF ↓
C3Molecular dynamics simulations of membrane electroporationPDF ↓
E1Electroporation of cells and tissues — interactive e-learningPDF ↓

Abstracts & publication

Abstract submission & proceedings

Participants were encouraged to submit a manuscript based on their abstract for a special issue of the Journal of Membrane Biology devoted to electroporation-based technologies and treatments, guest-edited by P. T. Vernier, L. M. Mir and D. Miklavčič.

EBTT 2009 proceedings cover

Electronic proceedings — all abstracts and course material.

Download proceedings (PDF)

Getting here & staying

Venue & accommodation

Held at the Faculty of Electrical Engineering, University of Ljubljana. Faculty floorplan (PDF) · Map of Ljubljana centre

Hotel Slon

Hotel Slon

City centre — registration & welcome drink venue, by Kongresni trg

Pension Mrak

Pension Mrak

Near Kongresni trg

University guest house

University guest house

Dom podiplomcev — Gostišče Jamnik, Cesta na Vrhovce 4

Student dormitory

Student dormitory

Dijaški dom Vič, Gerbičeva 51a

Support

Sponsors & partners

IGEA Iskra Medical BIA Separations Kemomed GFT of University of Bielefeld Bioelectrochemical Society Slovenian Research Agency (ARRS) CNRS CliniGene

Contacts

Organisation & contacts

Registration, abstract submission & other

Damijan Miklavčič
Faculty of Electrical Engineering, University of Ljubljana
Tržaška 25, 1000 Ljubljana, Slovenia
damijan.miklavcic@fe.uni-lj.si
tel +386 1 4768 456 · fax +386 1 4264 658

Accommodation & travel

Congress Agency Albatros
ksenija@albatros-bled.com
tel +386 4 5780 358 · fax +386 4 5780 355

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