Electroporation-Based Technologies and Treatments — International Scientific Workshop & Postgraduate Course
The meeting
Cell membrane electroporation, its mechanisms and applications in biology, medicine and biotechnology. The basis of the phenomena, current applications and future prospects were the main topics of this international meeting.
Whether you were a postgraduate student or just wanted to learn and contribute to the discussion, you were kindly invited to attend the workshop and the course on electroporation technologies and treatments. Abstract submission was strongly encouraged and was necessary if credits (5 ECTS) were sought. Each participant submitting an abstract also presented it in the form of a short oral presentation.
Organised by the University of Ljubljana and the Institute of Oncology, Ljubljana. Organising committee chair: Peter Kramar. Members: Alenka Maček Lebar, Maša Kandušer, Matej Kranjc, Tadej Kotnik, Gorazd Pucihar, Mojca Pavlin, Nataša Pavšelj, Anže Županič.
Course content
D. Miklavčič — resting & induced transmembrane voltage; dielectric properties; temperature & conductivity changes.
E. Neumann — electrochemical membrane thermodynamics; transport mechanisms.
L. M. Mir — electroporation of cells in vitro and in vivo; efficiency parameters.
D. Miklavčič — devices & electrodes for in vitro/in vivo; tissue models and their validation.
G. Serša — mechanisms, preclinical & clinical use, future perspectives.
J. Teissié — gene transfection in vitro/in vivo; siRNA electrotransfer; veterinary & human clinical use.
V. Préat — skin electroporation; physical methods for DNA delivery into skin.
Nanosecond pulses; safety & toxicity of in-vivo EP; non-thermal irreversible electroporation; food sanitization; nanoelectropores; MD simulations of lipid bilayer electroporation.
Faculty
Programme & schedule
Hands-on
Wet-lab, computer-modelling and e-learning sessions. Participants could select up to three practical-work sessions. Exercise sheets (PDF) as distributed to participants. See also the practical-work assignment form.
| S1 | Laboratory safety | PDF ↓ |
| L1 | Gene electrotransfer with different pulse parameters | PDF ↓ |
| L2 | Monitoring cell membrane electroporation with ratiometric fluorescent dye Fura-2AM | PDF ↓ |
| L3 | Electropermeabilization detection of attached cells with propidium iodide | PDF ↓ |
| L4 | Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS | PDF ↓ |
| L5 | Electroporation of planar lipid bilayers | PDF ↓ |
| L6 | Cell electrofusion | PDF ↓ |
| C1 | Electrochemotherapy treatment planning: optimization of voltage & electrode position | PDF ↓ |
| C2 | Numerical modelling of coupled physical phenomena | PDF ↓ |
| C3 | Molecular dynamics simulations of membrane electroporation | PDF ↓ |
| E1 | Electroporation of cells and tissues — interactive e-learning | PDF ↓ |
Abstracts & publication
Participants were encouraged to submit a manuscript based on their abstract for a special issue of the Journal of Membrane Biology devoted to electroporation-based technologies and treatments, guest-edited by P. T. Vernier, L. M. Mir and D. Miklavčič.
Electronic proceedings — all abstracts and course material.
Download proceedings (PDF)Getting here & staying
Held at the Faculty of Electrical Engineering, University of Ljubljana. Faculty floorplan (PDF) · Map of Ljubljana centre

City centre — registration & welcome drink venue, by Kongresni trg

Near Kongresni trg

Dom podiplomcev — Gostišče Jamnik, Cesta na Vrhovce 4

Dijaški dom Vič, Gerbičeva 51a
Contacts